Peter Parker/Spider-Man (voice)
Josh Keaton
Gwen Stacy (voice)
Lacey Chabert
J. Jonah Jameson (voice)
Daran Norris
Flash Thompson (voice)
Joshua LeBar
Otto Octavius/Doctor Octopus (voice)
Peter MacNicol
Liz Allen (voice)
Alanna Ubach
Sally Avril (voice), Betty Brant (voice)
Grey DeLisle
Alex O'Hirn/Rhino (voice)
Clancy Brown
Harry Osborn (voice)
James Arnold Taylor
Norman Osborn (voice)
Alan Rachins
Aunt May Parker (voice)
Deborah Strang
Eddie Brock (voice)
Ben Diskin
Curt Connors/Lizard (voice)
Dee Bradley Baker
Adrian Toomes/Vulture (voice)
Robert Englund
Max Dillon/Electro (voice)
Crispin Freeman
Montana/Shocker (voice)
Jeff Bennett
Director
Victor Cook
Executive Producer
Stan Lee
Writer
Kevin Hopps
Dave Bullock
Producer
Greg Weisman
Troy Adomitis
Character Designer
José Zelaya